原標(biāo)題：IgE和T細(xì)胞對(duì)草花粉第5組主要變應(yīng)原Phl p 5的識(shí)別情況剖析
　?、偬菽敛莼ǚ壑饕^敏原Phl p 5是花粉熱和哮喘最強(qiáng)的過敏原之一。②本研究對(duì)Phl p 5的免疫優(yōu)勢IgE-和T細(xì)胞識(shí)別位點(diǎn)進(jìn)行了研究。③合成了7個(gè)肽段，P1 - P7全長31 - 38個(gè)氨基酸，跨越Phl p 5序列，采用圓二色譜法對(duì)其進(jìn)行了表征，并對(duì)IgE反應(yīng)活性、嗜堿性細(xì)胞活化和T細(xì)胞反應(yīng)活性進(jìn)行了測試。研究了載體結(jié)合肽誘導(dǎo)兔IgG抗體的能力，該抗體能識(shí)別不同草種的Phl p 5或交叉反應(yīng)性變應(yīng)原。檢測肽特異性抗體抑制IgE對(duì)Phl p 5的反應(yīng)活性的能力，以及在過敏患者由過敏原引起的嗜堿性細(xì)胞活化的能力。④肽段無二級(jí)結(jié)構(gòu)，無IgE反應(yīng)活性或相關(guān)致敏活性，表明Phl p 5 IgE表位具有構(gòu)象性。除P3外，肽段特異性IgG抗體阻斷了變態(tài)反應(yīng)性和與溫帶禾草發(fā)生交叉反應(yīng)的phlp5與IgE的結(jié)合。IgE抑制實(shí)驗(yàn)和分子建模在phlp5的N-和c -末端區(qū)域發(fā)現(xiàn)了幾個(gè)聚集構(gòu)象的IgE表位。P4在患者中刺激T細(xì)胞和細(xì)胞因子的反應(yīng)最強(qiáng)，但不屬于主要的IgE反應(yīng)區(qū)域。⑤我們的研究顯示了一個(gè)有趣的現(xiàn)象：Phl p 5中的IgE-和T細(xì)胞反應(yīng)域是分離的，這為開發(fā)新的免疫療法提供了基礎(chǔ)，這種療法可以選擇性地針對(duì)IgE或T細(xì)胞的反應(yīng)。


延伸閱讀
JACI
Dissection of the IgE and T-cell recognition of the major group 5 grass pollen allergen Phl p 5
DOI: https://doi.org/10.1016/j.jaci.2013.08.038
Background
The major timothy grass pollen allergen Phl p 5 belongs to the most potent allergens involved in hay fever and asthma.
Objective
This study characterized immune-dominant IgE- and T-cell–recognition sites of Phl p 5.
Methods
Seven peptides, P1 to P7 with a length of 31 to 38 amino acids that spanned the Phl p 5 sequence, were synthesized, characterized by circular dichroism spectroscopy, and tested for IgE reactivity, basophil activation, and T-cell reactivity. Carrier-bound peptides were studied for their ability to induce IgG antibodies in rabbits which recognize Phl p 5 or cross-reactive allergens from different grass species. Peptide-specific antibodies were tested for the capability to inhibit IgE reactivity to Phl p 5 and allergen-induced basophil activation of patients with allergy.
Results
The peptides exhibited no secondary structure and showed no IgE reactivity or relevant allergenic activity, indicating that Phl p 5 IgE epitopes are conformational. Except for P3, peptide-specific IgG antibodies blocked IgE binding to Phl p 5 of patients with allergy and cross-reacted with temperate grasses. IgE inhibition experiments and molecular modeling identified several clustered conformational IgE epitopes on the N- as well as C-terminal domain of Phl p 5. P4, which stimulated the strongest T-cell and cytokine responses in patients, was not part of the major IgE-reactive regions.
Conclusion
Our study shows an interesting dissociation of the major IgE- and T-cell–reactive domains in Phl p 5 which provides a basis for the development of novel forms of immunotherapy that selectively target IgE or T-cell responses.
All Authors:
Margarete Focke-Tejkl Raffaela Campana Renate Reininger Christian Lupinek Katharina Blatt Peter Valent Tea Pavkov-Keller Walter KellerWalter KellerRudolf Valenta
　　創(chuàng)建過敏性疾病的科研、科普知識(shí)交流平臺(tái)，為過敏患者提供專業(yè)診斷、治療、預(yù)防的共享平臺(tái)。