原標(biāo)題：Der p 基因組和轉(zhuǎn)錄組的高質(zhì)裝配揭示了多種新的塵螨過敏原
　?、傥輭m螨(HDM)是吸入性過敏原的主要來源，占全球過敏病例的50%以上，而屋塵螨過敏原的全譜尚不清楚。在這里，我們測序了一個高質(zhì)量的戶塵螨（Der p）基因組，以發(fā)現(xiàn)已知的典型過敏原和從粉塵螨在（Der f）基因組衍生出的過敏原同源物。②采用PacBio、Illumina和離子激流讀的混合組裝方法組裝了戶塵螨（Der p）基因組。用Cufflinks比較Der p和Der f 的轉(zhuǎn)錄組。采用二維凝膠電泳法和MALDI-ToF MS鑒定過敏原，通過ELISA法用病人血清與細(xì)菌表達(dá)的重組蛋白結(jié)合的方法檢測新過敏原的變應(yīng)原性。③基因組學(xué)、轉(zhuǎn)錄組學(xué)和蛋白質(zhì)組學(xué)方法揭示了21種已知過敏原的完整基因結(jié)構(gòu)，并揭示了11種假定的過敏原同源物。構(gòu)建了66.8 Mb的高質(zhì)Der p 基因組。該基因組組合估計(jì)占基因組大小的98.2%，其中重疊群N50為80kb。第一個全面的Der p和Der f轉(zhuǎn)錄組分析揭示了D. pteronyssinus and D. farinae兩種塵螨獨(dú)特的過敏原基因表達(dá)，其中,Der p 1、5、10、21和24在 d . pteronyssinus中高度表達(dá),而Der f 10, 13,21,2至31在d . farinae表達(dá)更高。ELISA檢測，Der p25、26、28、32、33的IgE結(jié)合率為38.5-65.3%，其中Der p33的過敏原性最高。④Der p基因組和過敏原的生物信息學(xué)和實(shí)驗(yàn)表征為今后HDM過敏原的研究提供了重要的資源
延伸閱讀
JACI
[IF:13.1]
High-quality assembly of Dermatophagoides pteronyssinus genome and transcriptome reveals a wide range of novel allergens
DOI: https://doi.org/10.1016/j.jaci.2017.12.910
 Abstract:
Rationale
House dust mites (HDM) are a predominant source of inhalant allergens that attribute to over 50% of worldwide allergy cases, while the full spectrum of HDM allergens remains unknown. Here we sequenced a high-quality genome of Dermatophagoides (D.) pteronyssinus to find known canonical allergens and allergen orthologs inferred from D. farinae genome.
Methods
The D. pteronyssinus genome was assembled by a hybrid assembly approach using PacBio, Illumina and Ion Torrent reads. Transcriptomes of D. pteronyssinus and D. farinae were compared using Cufflinks. Allergens were identified by two-dimensional gel electrophoresis and MALDI-ToF MS. Allergenicities of the novel allergens were detected by ELISA using patients’ sera against bacteria expressing recombinant proteins.
Results
Genomic, transcriptomic, and proteomic approaches revealed full gene structures of 21 known allergens, and uncovered 11 putative allergen homologs. A high-quality D. pteronyssinus genome of 66.8 Mb was constructed. This genome assembly represented 98.2% of estimated genome size, with contig N50 being 80kb. The first comprehensive transcriptomic analysis of D. pteronyssinus and D. farinae revealed distinctively expressed allergen genes between the two dust mites, in which Der p 1, 5, 10, 21 and 24 were highly expressed in D. pteronyssinus, while Der f 10, 13, 21, 26 and 31 expressed higher in D. farinae. From ELISA, IgE binding rates of Der p 25, 26, 28, 32, and 33 were 38.5-65.3%, in which Der p 33 had the highest allergenicity.
Conclusions
Bioinformatics and experimental characterizations of D. pteronyssinus genome and allergens provide important resources for future investigation of HDM allergens
All Author:
Angel Tsz-Yau Wan  Xiao-Yu Liu Kevin Yi Yang Mingqiang Wang Jamie Sui-Lam Kwok Zhiyuan Yang Xiao-Jun Xiao Carol Po-Ying Lau  Ying Li Zhi-ming Huang Jin-ge Ba Aldrin Kay-Yuen Yim Chun-Yan Ouyang Sai-Ming Ngai x
Sai-Ming Ngai
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Ting-Fung Chan Elaine Lai-Han Leung x
Elaine Lai-Han Leung
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Liang Liu Zhi-Gang Liu Stephen Kwok-Wing Tsui
　　創(chuàng)建過敏性疾病的科研、科普知識交流平臺，為過敏患者提供專業(yè)診斷、治療、預(yù)防的共享平臺。