原標(biāo)題：出生后18個(gè)月鼻腔微生物群的建立:與早期鼻炎和喘息的關(guān)系
　　①早期鼻腔微生物群的動(dòng)態(tài)建立影響局部粘膜免疫反應(yīng)和兒童呼吸道疾病的易感性。②本病例對照研究的目的是監(jiān)測、評(píng)估和比較患有鼻炎和喘息的嬰兒在出生后18個(gè)月與健康對照組嬰兒鼻微生物群的發(fā)育情況。③對122名新加坡健康成長(GUSTO)出生隊(duì)列受試者的前鼻拭子進(jìn)行了為期18個(gè)月的7個(gè)時(shí)間點(diǎn)的縱向收集。采用16S rRNA多路雙端測序法對3組臨床患者(1)單純鼻炎患者(28例)、鼻炎伴喘息患者(34例)和健康對照組(60例)進(jìn)行鼻微生物區(qū)系特征分析。④與對照組相比，兩組嬰兒鼻炎組的鼻腔微生物群的成熟都遵循獨(dú)特的模式。對照組患兒18個(gè)月期間細(xì)菌多樣性增加，而鼻炎患兒呈下降趨勢(P < 0.05)。增加豐富的Oxalobacteraceae家庭(變形菌門門)和Aerococcaceae家庭(硬壁菌門菌門)與鼻炎和伴隨的喘息(P < . 01),而調(diào)整棒狀桿菌科家族(放線菌門)和早期殖民Staphylococcaceae家庭(厚壁菌門門;與對照組(調(diào)整后P < 0.05)比較，差異有統(tǒng)計(jì)學(xué)意義(P < 0.05)。鼻炎組與對照組的唯一區(qū)別是棒狀桿菌科的豐度降低(P < 0.05)。決定鼻腔微生物群演替的因素包括性別、分娩方式、是否有兄弟姐妹、是否照顧嬰兒。們的研究結(jié)果支持了鼻部微生物群參與嬰兒早期鼻炎和喘息發(fā)展的假說。
延伸閱讀
JACI                                                          
[IF:13.1]
Establishment of the nasal microbiota in the first 18 months of life: Correlation with early-onset rhinitis and wheezing
DOI: https://doi.org/10.1016/j.jaci.2018.01.032
 Abstract:
Background
Dynamic establishment of the nasal microbiota in early life influences local mucosal immune responses and susceptibility to childhood respiratory disorders.
Objective
The aim of this case-control study was to monitor, evaluate, and compare development of the nasal microbiota of infants with rhinitis and wheeze in the first 18 months of life with those of healthy control subjects.
Methods
Anterior nasal swabs of 122 subjects belonging to the Growing Up in Singapore Towards Healthy Outcomes (GUSTO) birth cohort were collected longitudinally over 7 time points in the first 18 months of life. Nasal microbiota signatures were analyzed by using 16S rRNA multiplexed pair-end sequencing from 3 clinical groups: (1) patients with rhinitis alone (n = 28), (2) patients with rhinitis with concomitant wheeze (n = 34), and (3) healthy control subjects (n = 60).
Results
Maturation of the nasal microbiome followed distinctive patterns in infants from both rhinitis groups compared with control subjects. Bacterial diversity increased over the period of 18 months of life in control infants, whereas infants with rhinitis showed a decreasing trend (P < .05). An increase in abundance of the Oxalobacteraceae family (Proteobacteria phylum) and Aerococcaceae family (Firmicutes phylum) was associated with rhinitis and concomitant wheeze (adjusted P < .01), whereas the Corynebacteriaceae family (Actinobacteria phylum) and early colonization with the Staphylococcaceae family (Firmicutes phylum; 3 weeks until 9 months) were associated with control subjects (adjusted P < .05). The only difference between the rhinitis and control groups was a reduced abundance of the Corynebacteriaceae family (adjusted P < .05). Determinants of nasal microbiota succession included sex, mode of delivery, presence of siblings, and infant care attendance.
Conclusion
Our results support the hypothesis that the nasal microbiome is involved in development of early-onset rhinitis and wheeze in infants.
All Author:
Le Duc Huy Ta Gaik Chin Yap Carina Jing Xuan Tay Alicia Shi Min Lim Chiung-Hui Huang
Collins Wenhan Chu Paola Florez De Sessions Lynette P. Shek Anne Goh Hugo P.S. Van Bever Oon Hoe Teoh Jian Yi Soh Biju Thomas Mahesh Babu Ramamurthy Daniel Y.T. Goh Christophe Lay Shu-E Soh Yiong Huak Chan Seang-Mei Saw Kenneth Kwek  Yap-Seng Chong Keith M. Godfrey Martin Lloyd Hibberd , Bee Wah Lee xMartin Lloyd HibberdSearch for articles by this author
Affiliations
· Genome Institute of Singapore, Agency for Science, Technology and Research Singapore, Singapore
　　創(chuàng)建過敏性疾病的科研、科普知識(shí)交流平臺(tái)，為過敏患者提供專業(yè)診斷、治療、預(yù)防的共享平臺(tái)。