原標題：早期腸道微生物構(gòu)成與牛奶過敏的消除
　　腸道微生物群可能在牛奶過敏的自然病中起作用。
　　我們試圖研究早期腸道微生物群與牛奶過敏之間的關(guān)系。
　　我們研究了226名牛奶過敏的兒童，他們在嬰兒期就被納入食物過敏觀察研究組。收集3 - 16個月時的糞便樣本，對患兒進行縱向隨訪，進行臨床評估、牛奶特異性IgE水平、入組時、6個月、12個月、之后每年進行一次，直到8歲。腸道微生物組采用16s rRNA測序，微生物組分析采用微生物生態(tài)學定量研究(QIIME)、群落系統(tǒng)發(fā)育研究(PICRUSt)和元基因組譜統(tǒng)計分析(STAMP)。
　　226名兒童中128名(56.6%)的牛奶過敏在8歲時消失。3 - 6個月時的腸道菌群組成與8歲時牛奶過敏反應的消除有關(guān)(PERMANOVA P = .047)，牛奶過敏問題的解決與嬰兒腸道菌群中梭菌和厚壁菌的富集有關(guān)。元基因組功能預測支持牛奶過敏消除的受試者中道微生物組脂肪酸代謝的減少 (η2 = 0.43;方差分析P = .034)。
　　嬰兒早期是一個窗口，在此期間腸道微生物群可能影響兒童食物過敏的結(jié)果。梭狀芽胞桿菌和厚壁菌綱中的細菌類群可作為乳過敏治療的益生菌候選物進行研究。
　　延伸閱讀


Clin Exp Allergy 
Early-life gut microbiome composition and milk allergy resolution
DOI: 10.1016/j.jaci.2016.03.041
Abstract:
Background
Gut microbiota may play a role in the natural history of cow's milk allergy.
Objective
We sought to examine the association between early-life gut microbiota and the resolution of cow's milk allergy.
Methods
We studied 226 children with milk allergy who were enrolled at infancy in the Consortium of Food Allergy observational study of food allergy. Fecal samples were collected at age 3 to 16 months, and the children were followed longitudinally with clinical evaluation, milk-specific IgE levels, and milk skin prick test performed at enrollment, 6 months, 12 months, and yearly thereafter up until age 8 years. Gut microbiome was profiled by 16s rRNA sequencing and microbiome analyses performed using Quantitative Insights into Microbial Ecology (QIIME), Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt), and Statistical Analysis of Metagenomic Profiles (STAMP).
Results
Milk allergy resolved by age 8 years in 128 (56.6%) of the 226 children. Gut microbiome composition at age 3 to 6 months was associated with milk allergy resolution by age 8 years (PERMANOVA P = .047), with enrichment of Clostridia and Firmicutes in the infant gut microbiome of subjects whose milk allergy resolved. Metagenome functional prediction supported decreased fatty acid metabolism in the gut microbiome of subjects whose milk allergy resolved (η2 = 0.43; ANOVA P = .034).
Conclusions
Early infancy is a window during which gut microbiota may shape food allergy outcomes in childhood. Bacterial taxa within Clostridia and Firmicutes could be studied as probiotic candidates for milk allergy therapy.
All Authors:
SupindaBunyavanichMD,MPHabNanShenMSaAlexanderGrishinPhDbRobertWoodMDcWesleyBurksMDdPeterDawsonPhDeStacie M.JonesMDfDonald Y.M.LeungMD, PhDgHughSampsonMDbScottSichererMDbJose C.ClementePhDah
　　創(chuàng)建過敏性疾病的科研、科普知識交流平臺，為過敏患者提供專業(yè)診斷、治療、預防的共享平臺。