原標(biāo)題：用來自全球主要螨蟲種類的純化和重組過敏原組份診斷螨蟲過敏
　　①螨蟲是過敏性疾病最重要的過敏原來源之一。雖然免疫療法是唯一可以緩解這些癥狀的治療方式，但用于此類目的的過敏性提取物并沒有明確的定義。②本研究的目的是評(píng)估在兩種不同環(huán)境(新加坡和意大利)的變態(tài)反應(yīng)診所就診的一系列患者的IgE結(jié)合譜，采用IgE免疫印跡法，使用了100多種不同的純化天然或重組過敏原，這些過敏原來自世界各地的主要螨蟲種類。這可能有助于選用適當(dāng)?shù)闹苿┲委焿m螨和/或熱帶無爪螨過敏者的免疫治療，也可以用于監(jiān)測(cè)正在接受治療的患者。③多數(shù)塵螨致敏患者能用第1、2、4、5、7和13聯(lián)合組份進(jìn)行診斷，而熱帶無爪螨過敏者可以用有rBlo t 5、rBlo t 5同源、 rBlo t 13、rBlo t 13同源、Blo t 3和Blo t 12。然而，第10組(原肌球蛋白)塵螨過敏原與其他幾種原肌球蛋白過敏原(如海鮮或蟑螂)的致敏無關(guān)。④結(jié)論:我們可以從致敏程度較廣的患者中鑒別出對(duì)主要塵螨變應(yīng)原(第1、2組)敏感的螨蟲過敏患者。在塵螨為主地區(qū)(如意大利)和以熱帶無爪螨為主的地區(qū)(如新加坡)，需要用不同的過敏原來診斷患者。
延伸閱讀
JACI:
Component-resolved diagnosis of house dust mite allergy with a large repertoire of purified natural and recombinant allergens from the major species of mites worldwide
DOI: https://doi.org/10.1016/j.jaci.2004.12.667
Abstract:
RATIONALE: Mites are one the most important sources of allergens associated with allergic diseases. Although immunotherapy is the only form of treatment that can alleviate these conditions, allergenic extracts used for such purposes are not well defined.
METHODS: The aim of this study was to evaluate the IgE binding profile of a series of patients attending allergy clinics in two different environments (Singapore and Italy) by IgE immuno-blotting using an arrayed panel of more than 100 different purified natural or recombinant allergens from major species of mites worldwide. This may contribute to improved selection of patients for immunotherapy with Dermatophagoides spp. and/or Blomia tropicals sensitization and may also be used for monitoring of patients undergoing treatment.
RESULTS: Majority of patients with sensitization to Dermatophagoides spp. could be diagnosed with a combination of the group 1, 2, 4, 5, 7 and 13 allergens while those with sensitization to Blomia tropicalis could be diagnosed with a combination of rBlo t 5, rBlo t 5 paralogue, rBlo t 13, rBlo t 13 paralogue, Blo t 3 and Blo t 12. The group 10 (tropomyosin) mite allergens however did not correlate with sensitization to several other tropomyosin allergens e.g., from seafood or cockroach.
CONCLUSIONS: We could discriminate mite-allergic patients who were mainly sensitized to the major Dermatophagoides allergens (group 1 and 2) from patients with broad sensitization. Different allergens are needed to diagnosed patients found in Dermatophagoides-predominant regions (e.g., Italy) and where Blomia tropicalis is predominant (e.g., Singapore).
All Author:
A.S.L. Tay, H.S. Shang, X.Z. Bi, K. Reginald, Y.F. Gao, S.T. Ong, A.C. Angus, W.L. Wang, K.T. Kuay, D.Y. Wang, A. Mari, F.T. Chew
　　創(chuàng)建過敏性疾病的科研、科普知識(shí)交流平臺(tái)，為過敏患者提供專業(yè)診斷、治療、預(yù)防的共享平臺(tái)。