原標(biāo)題：特應(yīng)性皮炎患者對(duì)各種屋塵螨過(guò)敏原中次要組分的敏感性高于呼吸道過(guò)敏性疾病患者
　?、偈覂?nèi)塵螨(HDM)可產(chǎn)生多種致敏蛋白。然而，這些過(guò)敏原的致敏性和臨床意義尚不清楚。②本研究的目的是確定粉塵螨過(guò)敏原，并闡明韓國(guó)患有呼吸道(變應(yīng)性鼻炎和/或哮喘)和特應(yīng)性皮炎癥狀的患者對(duì)這些過(guò)敏原的敏感性情況。③對(duì)160例HDM過(guò)敏患者血清IgE反應(yīng)性進(jìn)行單、二維凝膠電泳和免疫印跡分析。IgE‐活性組分采用液相色譜-耦合電噴霧電離-串聯(lián)質(zhì)譜法測(cè)定。產(chǎn)生9種重組螨變應(yīng)原(Der f1、Der f2、Der f10、Der f11、Der f13、Der f14、Der f30、Der f32、Der f Alt a 10)， ELISAs測(cè)定血清中IgE對(duì)上述組份的反應(yīng)活性。④ Der f1和Der f2分別被88.1%和78.1%患者的血清IgE識(shí)別。呼吸道過(guò)敏患者主要對(duì)這兩種主要過(guò)敏原敏感，特應(yīng)性皮炎患者對(duì)主要和次要過(guò)敏原成分(包括Der f11、Der f13、Der f14、Der f32和Der falt a10)表現(xiàn)出多重致敏性。⑤呼吸道過(guò)敏性疾病患者對(duì)HDM的主要過(guò)敏原組份敏感。特應(yīng)性皮炎患者對(duì)HDM中更廣泛的次要過(guò)敏原組份(Der f11、Der f13、Der f14、Der f32和Der falt a10)敏感。


延伸閱讀
Clinical & Experimental Allergy：
Sensitization to various minor house dust mite allergens is greater in patients with atopic dermatitis than in those with respiratory allergic disease
https://doi.org/10.1111/cea.13164
Background
Various allergenic proteins are produced by house dust mites (HDM). However, the allergenicity and clinical implications of these allergens are unknown.
Objective
The purpose of this study was to identify allergens in Dermatophagoides farinae and elucidate the sensitization profiles to these in Korean patients suffering from respiratory (allergic rhinitis and/or asthma) and atopic dermatitis symptoms.
Methods
IgE reactivities in sera from 160 HDM allergy patients were analysed by one‐ and two‐dimensional gel electrophoresis and immunoblotting. IgE‐reactive components were identified by liquid chromatography‐coupled electrospray ionization‐tandem mass spectrometry. Nine recombinant mite allergens (Der f 1, Der f 2, Der f 10, Der f 11, Der f 13, Der f 14, Der f 30, Der f 32 and Der f Alt a 10) were produced, and the IgE reactivity in sera to each was determined by ELISAs.
Results
Der f 1 and Der f 2 were recognized by IgE in serum samples from 88.1% and 78.1% of all patients, respectively. Patients with respiratory allergies were mainly sensitized to these major allergens, whereas patients with atopic dermatitis symptoms showed polysensitization to major and minor allergen components (including Der f 11, Der f 13, Der f 14, Der f 32 and Der f Alt a 10).
Conclusions
Patients with respiratory allergic disease sensitize to major allergen components of HDM. Those with atopic dermatitis were sensitized to a broader range of minor allergen components of HDM (Der f 11, Der f 13, Der f 14, Der f 32 and Der f Alt a 10).
All Authors
K. H. Park J. Lee J.‐Y. Lee S. C. LeeD. W. SimJ. U. ShinC. O. ParkJ.‐H. LeeK. H. LeeK. Y. Jeong
　　創(chuàng)建過(guò)敏性疾病的科研、科普知識(shí)交流平臺(tái)，為過(guò)敏患者提供專業(yè)診斷、治療、預(yù)防的共享平臺(tái)。